The facility houses two Real-Time PCR Instruments.

The Applied Biosystems 7500 Real Time PCR Systems uses fluorescent-based PCR chemistries to provide quantitative detection of nucleic acid sequences using real-time analysis and qualitative detection of nucleic acid sequences using end-point and association-curve analysis.

Run time of less than 2 hours

Variable excitation and five-color detection enable you to use a broad range of fluorophores including FAM™/SYBR® Green I, VIC®/JOE, NED™/ TAMRA™/ Cy3™, ROX™/Texas Red®, and Cy5™ dyes for greater assay versatility.

Measure gene expression levels , detect and quantitate pathogens, perform allelic discrimination (SNP genotyping) assays as well as score the presence of gene sequences

Supports 96-well format plates and 0.2 mL tubes

Powerful, versatile software including plate set-up wizards and automated analysis tools make data processing simple and straightforward.

STANDARD PRICES:

Charge per run $15.00

Reagents for both instruments are available through the supply center.

For any questions please contact the DNA Sequencing lab by calling (973) - 972 - 2625 or by emailing mrfadm@njms.rutgers.edu

MRF Instrument Registration

RT-PCR AB7500

Instrument 1 - Schedule Calendar [DO NOT USE. TESTING]

If you would like to sign up for an open slot, please click on this link and fill out the form. [DO NOT USE..BEING TESTED]